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Promega gsh gssg

WebGSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader. The assay is based on the glutathione recycling system by DTNB and glutathione reductase. WebJun 7, 2024 · One microgram of cDNA was used to detect various spliced forms of Xbp-1 using GoTaq DNA polymerase (#M3001, Promega, Madison, WI, USA). The PCR amplification protocol was set to 10 min at 95 °C, followed by 1 min each at 95 °C, 59 °C, and 72 °C for 50 cycles, and final elongation at 72 °C for 10 min. cDNA (5 ng) was used for …

GSH/GSSG-Glo™ Assay Protocol - promega.jp

WebJun 7, 2024 · One microgram of cDNA was used to detect various spliced forms of Xbp-1 using GoTaq DNA polymerase (#M3001, Promega, Madison, WI, USA). The PCR … WebThe extract caused increase in GSH/GSSG ratio, yet it exacerbated the decrease in glutathione content in the UVB-exposed cells. These effects of the laver extract were also manifested in the sham-exposed cells, suggesting that those effects might be general phenomena caused by the laver extract. floating suction line https://bernicola.com

Glutathione Assay Kit - Cayman Chem

Web1.1.2 酶和试剂 人源溶菌酶标准品购买于北京经科宏达生物技术有限公司；NdeⅠ和XhoⅠ限制性核酸内切酶购买于日本TaKaRa公司；T4 DNA连接酶购买于美国Promega公司；DNA Marker 5000购买于康为世纪生物科技有限公司；琼脂糖、尿素、DTT、EDTA、Triton X-100、IPTG、PMSF购买于 ... WebAug 5, 2024 · Product Specs Item GSH/GSSG-Glo Assay Company Promega Price Supplier Page View Company Product Page Catalog Number V6611 Quantity 10ml Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (72) WebAfter treatment of 10 μM NR1 for 72 h, cells (1×10 6 per sample) were lysed in 100 μL of lysis buffer to perform the GSH/GSSG assay. The GSH/GSSG assay was performed using the Green Fluorometric Detection Kit (AAT Bioquest), which uses a proprietary nonfluorescent dye that becomes strongly fluorescent upon reacting with GSH (Ex/Em =490/520 nm). great lakes cargo ships

N-acetylcysteine overcomes NF1 loss-driven resistance to PI3Kα ...

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Promega gsh gssg

Promega GSH/GSSG-Glo™ Glutathione Assay - Fisher Sci

WebPromega GSH/GSSG-Glo™ Glutathione Assay. The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH … Web2 or 100 μM DEM for 6 h, and reduced GSH levels were determined using GSH-Glo assay (Promega) (n = 3). (F)3T3KRASV12, EN, and MSCV cells were treated with 200 μMH 2O 2 or 100 μM DEM for 6 h, and GSH/GSSG ratio was determined using GSH/ GSSG-Glo assay (Promega) (n = 3).

Promega gsh gssg

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WebApr 18, 2024 · Interestingly, while 3T3 KRAS V12 and EN cells exhibited a lower GSH/GSSG ratio relative to 3T3 MSCV cells under ambient conditions, these cells had similar GSH/GSSG ratios as 3T3 MSCV cells following H 2 O 2 treatment ( Fig. 1 F ). WebPromega GSH/GSSG-Glo™ Glutathione Assay The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. Manufacturer: Promega V6611 View more versions of this product Catalog No. PRV6611 $743.50 / Each of 1 Qty Check …

WebMethyl mercury (MeHg) is an organic highly toxic compound that is transported efficiently via the human placenta. Our previous data suggest that MeHg is taken up into placental cells by amino acid transporters while mercury export from placental WebOct 5, 2024 · Finally, the NADPH/NADP + and GSH/GSSG ratios were calculated to monitor the redox state of the cells. NADPH is a coenzyme widely involved in a broad range of …

WebPromega GSH/GSSG-Glo™ Glutathione Assay. The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH … WebFeb 6, 2014 · 本研究表 腹内压20mmHg作用1 h即可导致GSH的降 低以及GSSG的升高, GSH/GSSG比率随腹内压 力的增高及作用时间的延长显著下降. T-AOC测 定结果也显示肠黏膜组织总抗氧化能力随压力 增高逐渐下降, 至30 mmHg 总抗氧化能力比正常对照组下降了67%.

WebApr 14, 2024 · I GSH and GSSG levels in HyPer-DAO-NLS wt and C148A cells n.t. or after treatment with 50 mM D-ala or ... Glutathione levels were determined using the GSH/GSSG-Glo TM assay kit (V6611, Promega) ...

WebThe ratio between GSSG and GSH in cells is normaly 1:10 or even lower. GSH makes up 80% of free thiols in cells. Thus determination of free thiols can be used to measure GSH content... great lakes cargo vesselsWebSep 29, 2024 · GSH + GSSG levels and ROS production in LPS-treated cells with or without BSO. Cells were pretreated with 120 µM BSO for 24 h, followed by 10 ng/ml LPS for 2 h. Total GSH + GSSG and GSSG levels measured in the cell lysates are the mean ± SD of six biological replicates from three independent experiments and are expressed as … great lakes carpentryWebThe GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. A change in GSH levels is … floating suction skimmer australiaWebgsh/gssg-glo™ assay (gsh/gssg-glo™ 检测试剂盒) 是利用发光法检测和定量培养细胞中的总谷胱甘肽(gsh+gssg)、gssg以及gsh/gssg 比率的检测系统。gsh 水平的改变对细胞毒性 … great lakes caring home healthWebA Luminescent-Based Assay for the Detection and Quantification of Glutathione (GSH) Measure reduced (GSH) or total (GSSG + GSH) glutathione from cells and tissues. … great lakes carpetWebThe GSH/GSSG-Glo™ Assay is a luminescence-based system that detects and quantifies total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells. Reduced … GSH/GSSG-Glo™ Assay. Homogeneous assay to quantify total glutathione and … GSH/GSSG-Glo™ Assay. Homogeneous assay to quantify total glutathione and … The ApoLive-Glo™ Multiplex Assay measures both the number of viable cells … floating suction unitWebGSH, GSSG, activities of histone deacetylase (HDAC) and histone acetyltransferase, apoptosis, and phagocytosis were assessed in isolated AMs. great lakes car ferries