Phenolphthalein glucuronide

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August undefined, 2024

Webgins (1946) employing phenolphthalein mono-$-glucuronide as a chromogenic substrate is simple, sensitive and readily adaptable to histochemical work, and therefore it was chosen. Phenol- (Kerr et al., 1948) and p-chlorophenyl- (Spencer and Williams, 1951) glucuronides have also been employed as substrates, but WebFeb 19, 2024 · Application: Phenolphthalein Glucuronide, Sodium Salt is a useful substrate compound for β -glucuronidase assays CAS Number: 6820-54-8 Molecular Weight: 516.40 …

Phenolphthalein Sigma-Aldrich

WebSep 23, 2006 · Phenolphthalein HPLC Separation and Determination of Phenolphthalein and its Glucuronid as Markers of Enterohepatic Circulation Using Acetonitrile-Methanol as Organic Modifier and Application... Web∙ Activity Unit Definition: One unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 7.0 and 37°C. ∙ Sulfatase activity: ≥ 250,000 U/mL. ∙ Activity Unit Definition: One unit of sulfatase will hydrolyze 1.0 nmol of p-nitrocatechol sulfate per hour at pH 7.0 and 45°C. ravens wellness center

Phenolphthalein β-D-glucuronide - Sigma-Aldrich

WebJan 1, 2024 · Phenolphthalein Induces Thymic Lymphomas Accompanied by Loss of the p53 Wild Type Allele in Heterozygous p53-Deficient ( ) Mice Article Full-text available Nov 1997 June Dunnick Jerry Hardisty... WebBackground: Phenolphthalein is widely used as a safe and effective laxative. After oral administration, phenolphthalein is absorbed in the small bowel and is conjugated in the liver to phenolphthalein glucuronide which passes into the colon where it is deconjugated and the active compound, phenolphthalein, is released. WebEffective in the hydrolysis of steroid glucuronides. Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis Quality Contains buffer salts and stabilizer. Unit Definition One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay). ravenswell school bray

Phenolphthalein Request PDF - ResearchGate

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WebJul 24, 1992 · A procedure is described to simultaneously quantitate phenolphthalein and its glucuronide metabolite from dog serum, urine and bile using high-performance liquid chromatography. WebPhenolphthalein is absorbed in the small intestine and conjugated in the liver to form phenolphthalein glucuronide, which is eliminated in the bile. It is partially deconjugated and reabsorbed as it passes through the small intestine. The cathartic effect of the drug prolongs due to an entero-hepatic circulation (Sidhu et al., 1989 ). ravenswharfe hotelWebAlthough theoretically superior, phenolphthalein glucuronide was not found to be a more effective laxative compared to phenolphthalein in normal subjects. The therapeutic … simp copy and paste

"WebOct 4, 2008 · The phenolphthalein derivative is a previously characterised substrate for these enzymes [Citation 20], and in some cases the rate of its conversion was … " - Phenolphthalein glucuronide

Phenolphthalein glucuronide

Mammalian drug efflux transporters of the ATP binding cassette …

WebThe phenolphthalein mono+&glucuronide is prepared biosynthetically from the urine of rabbits to which phenolphthalein has been administered. This substrate is rap- idly hydrolyzed by /3-glucuronidase, and the free phenolphthalein may be readily determined photocolorimetrically in alkaline solution. WebApr 20, 2009 · Phenolphthalein is absorbed from the gastrointestinal tract and . undergoes extensive first-pass metabolism in the intestinal epithelium and liver, resulting in almost complete conversion to its glucuronide, which is eliminated in the bile (NTP 1999). Phenolphthalein enhances the production of oxygen radicals in . in vitro . systems (IARC …

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Webβ-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means. β-glucuronidase is used as a reporter gene in GUS … More Product Information … Web中国广东省深圳市坪山区 SCT创意工厂B1203 生命科技园 . 认证. ISO 9001:2015. 关注我们

WebAug 1, 2024 · The screening of β-glucuronidase is achieved on methylumbelliferyl glucuronide plate (MUG) which contain, 4-methylumbelliferyl glucuronide as substrate. The fluorescent 4-methylumbelliferyl... WebPhenolphthalein is an organic compound used as a laboratory reagent and pH indicator. Phenolphthalein exerts laxative effects by stimulating the intestinal mucosa and …

Web*One modified Fishman unit will liberate 1 microgram of phenolphthalein from phenolphthalein glucuronide per hour at 25°C at pH 6.8 (30-minute assay) TESTED ON REAL PATIENT URINE CONTAINING EXCESS GLUCURONIDES TO PRODUCE ACCURATE RESULTS. RAPID HYDROLYSIS BUFFER INCLUDED IN EVERY ORDER. WebSep 1, 2009 · A βG-specific probe was generated by labeling phenolphthalein glucuronide (PTH-G) with iodine 131 ( 131 I) or 124 I. To test the specificity of the probe in vitro, 124 I-PTH-G was added to CT26 and βG-expressing CT26 (CT26/βG) cells. Mice bearing CT26 and CT26/βG tumors ( n = 6) were injected with 124 I-PTH-G and subjected to micro-PET …

WebCat. No.: XGB907 Phenolphthalein β-D-glucuronide. Synonym: Phenolphthalein β-D-glucosiduronic acid,Phenolphthalein glucuronic acid,Phenolphthalein mono-β-D-glucosiduronic acid MDL: MFCD00135881 CAS Number: 15265-26-6 PubChem Substance ID: 24898108 REAXYS Number: 68526 NACRES: NA.32

http://bb-china.net/detailed.html?pid=35528 simpco portable \\u0026 steel buildingsWebPhenolphthalein β-Glucuronide Molecular Formula C26H22O10 • XH2O Formula Weight 494.5 Purity ≥98% Formulation(Request formulation change) A crystalline solid … simpco welding juneauWebThis biological-physicochemical hyphenation is able to detect and control active mechanisms of traditional medicines or botanicals as well as the essentials of plant-based food. The array of 1,292... ravenswey 4WebPhenolphthalein glucuronide (0.01 M; pH 5.0): Mix 0.837 g. cinchonidine salt with 20 ml. 2 N HCl, add 20 ml. ethyl acetate and stir until solution is complete. Transfer the mixture … ravens westryWebCiprofloxacin and phenolphthalein-β-d-glucuronide (PhePG), a typical β-glucuronidase substrate, significantly decreased the production of MPA from MPAG in the β-glucuronidase-mediated deconjugation system. In addition, enoxacin significantly inhibited the production of MPA from MPAG, while levofloxacin and ofloxacin had no inhibitory effect ... ravenswell roadWebAug 25, 2024 · The β-glucuronidase activity is measured by methylumbelliferyl glucuronide as substrate and when it is hydrolyzed, strong fluorescent methylumbelliferone is … simpcraft mrbeast ipWebPhenolphthalein, a drug used for most of the 20th century, was generally regarded as nontoxic and safe at therapeutic doses (approximately 1 to 2 mg/kg) ( NTP, 1996; IARC, … simpcw chief and council