WebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help … Webb24 mars 2024 · PPE-NC-v1 showed the highest editing efficiencies, ranging from 0.2% to 19.5% in both rice and wheat protoplasts, followed by PPE-NC-v2 and PPE-∆RNase H (Fig. 1f,g ). These three new PPEs...
High-efficiency prime editing with optimized, paired …
WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … Webb14 mars 2024 · After incubation at room temperature for 12 h, protoplasts were harvested and homogenized in 100 μl lysis buffer containing 25 mM Tris–HCl, pH 7.8, 2 mM DTT, 2 mM trans -1,2-diaminocyclohexane- N ’ N ’ N ’ N ’-tetraacetic acid, 10% (v/v) glycerol, 1% (v/v) Triton X-100. notfallpraxis pfaffenhofen
An engineered prime editor with enhanced editing efficiency in …
WebbPlasmid pH-A3A-PBE from Dr. Caixia Gao's lab contains the insert APOBEC3A-nCas9-NLS-UGI-NLS and is published in Nat Biotechnol. 2024 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. This plasmid is available through Addgene. WebbTotal vector size (bp) 6388 Vector type Plant Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Spectinomycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name BsaI:AATG:Cas9_2:GCTT:BsaI Species Synthetic Insert Size (bp) 4141 Tag / Fusion Protein WebbpH-nCas9-PPE-V2 Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about ... notfallpraxis preetz