Hepg2 contamination
Web11 apr. 2024 · Cell lines and cell culture. Human HCC cell lines MHCC-97H, HepG2, and HEK293T were obtained from the American Type Culture Collection. These cells were tested for mycoplasma contamination and cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (TIANHANG) and 1% … WebBisphenol A induces cholesterol biosynthesis in HepG2 cells via SREBP-2/HMGCR signaling pathway Qingrong Li, Hongmin Zhang, Jun Zou, Xiang Feng, Dan Feng J. Toxicol. ... Understanding dabs: contamination concerns of cannabis concentrates and cannabinoid transfer during the act of dabbing Jeffrey C. Raber, Sytze Elzinga, Charles Kaplan
Hepg2 contamination
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Web2 mei 2016 · As the number of reported deoxynivalenol (DON) contamination incidents increased steadily over the past decades, there has been a widespread interest in understanding the cellular mechanisms of the toxicological effects of DON using in vitro systems and omics technologies. The present investigation was conducted to … WebFigure 1 Western blotting (A) and associated statistical analysis (B) for the expression of E-cadherin (E-cad), SALL4 and CD90 in Chang liver cells (Chang), PLC/PRF/5 cells (PLC) and HepG2 cells (HepG2).The characters in this figure (a, b, c) indicate which percentages differ. Percentages with a same character are not statistically significant while …
Web22 nov. 2024 · After careful authentication of the HepG2 cell line used in our study, we believe the raised concerns regarding possible cell contamination and misusage can be resolved. During the study, cells … WebContamination was detected by fluorescence imaging (DAPI) and PCR reactions. The cultures were treated with BM-Cyclin antibiotic to eliminate contamination. The data …
Web1 nov. 2024 · The human hepatocellular carcinoma cell line HepG2, cultured in DMEM (containing 1% streptomycin/penicillin and 10% fetal bovine serum) at 37 °C in a humidified atmosphere containing 5% carbon dioxide, was provided by American Type Culture Collection (ATCC). WebHep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver …
WebAim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic...
WebHepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates … plasterboard wall adhesiveWeb15 apr. 2013 · HepG2 represents a pure cell line of human liver carcinoma, often used as HCC model due to the absence of viral infection. In this study we compare gene … plasterboard tv wall mountWeb16 mei 2006 · Human hepatocarcinoma cells, HepG2 were cultured onto biodegradable polyglycolic acid (PGA) polymer scaffolds, which were cultured in a rotating cell culture system (RCCS) to form a three-dimensional (3D) multicellular culture in vitro. The RCCS can simulate microgravity effects with low shear stress and well exchanging for gas. plasterbrain roblox idWebFor HepG2, 60mm dish, 80% confluency, 1-2min is enough. We have to use optimum trypsin, for optimum time for each cell line. Unless that gives clumps or debris. 4) What is … plasterboard wall thicknessplasterboard wall sectionWeb26 aug. 2024 · As shown in Figure 6 A,B, our results indicated that HepG2 cells had highly expressed ROS and that DHM could not scavenge ROS well at lower doses, while the intracellular delivery of DHM using ZIF-8 as a vector significantly affects the production of ROS in tumor cells, thus affecting tumor cell growth. Figure 6. plasterbrain face revealWebHepG2 and HCCLM3 were authenticated using short tandem repeat profiling AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit for high-resolution screening and interspecies cross-contamination detection. Lipofectamine-3000 (Invitrogen, USA) was employed for plasmids transfection according to the manufacturer’s protocol. Cell Proliferation Assay plasterbrain - nimbasa core lyrics