Flp-in t-rex
WebApr 6, 2024 · As most GBA1 mutations lead to a misfolded enzyme that is rapidly degraded via ER-associated degradation by the proteasome 11,12, we generated stable Flp-In™T-REx™-HEK 293 cell lines ... WebApr 21, 2024 · Flp-In t -293细胞系包含稳定整合的pFRT lacZeo和pcDNA 6 TR (来自T-REx系统)。 用Flp- in表达载体和Flp重组酶载体pOG44共转染Flp- in细胞系，可将表达载体定向整合到每个细胞的同一位点，保证基因 …
Flp-in t-rex
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WebInducible Flp-In T-Rex 293 cells were generated according to the manufacturer’s protocol (Thermo Fisher Scientific). The selection was performed with DMEM supplemented with 15 μg/ml blasticidin and 100 μg/ml hygromycin B Gold (InvivoGen) 48 h after transfection and continued until the expression of the gene of interest was induced by ... WebMay 17, 2011 · Flp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression...
WebMar 3, 2024 · Generation of Stable Inducible Cell Pools and BioID Labeling. Cell lines were generated in HEK293 Flp-In T-REx 293 (Invitrogen; used for BioID, affinity purification coupled to MS and some of the streptavidin-immunobot analyses) or HeLa Flp-In T-REx cells (used for BioID and the BioID-immunoblot analyses) grown at 37 °C in Dulbecco’s … WebUpon isolation of DPCs by PxP, samples were analysed using western blotting using the indicated antibodies. d HeLa T-REx Flp-In SPRTN-ΔC cells were complemented with YFP, YFP-SPRTN-Strep variants as indicated (wildtype (WT), E112Q (EQ), R185A (ZBD*)) and treated as shown in Fig. , before DPCs were isolated using PxP and analysed by …
WebApr 26, 2024 · Flp-In T-REx 293 cells expressing c-IBD-SBP-YAP1C were incubated in DPBS containing 10 mM 3-AT and supplemented or not with 1 mM H 2 O 2. After 10 min, free thiol groups were blocked with NEM, and the samples were processed as depicted in panel A and subsequently subjected to immunoblotting with rabbit pre-immune serum … WebMar 22, 2024 · Further investigation in differentiated HuH-7 cells expressing NTCP and NTCP-overexpressing Flp-In T-REx 293 cells revealed that the mechanism of action of everolimus on NTCP is direct inhibition and mTOR-independent. Structural analogs of everolimus inhibited NTCP-mediated TCA uptake, however, functional analogs did not …
Webjor components of the Flp-In™ T-REX™ System include: • A Flp-In™ target site vector, pFRT/lacZeo, for generation of a Flp-In™ host cell line containing an integrated FRT site. …
WebDescription. Once Flp-InT-REx host cell line containing integrated FRT site has been created, subsequent generation of Flp-InT-REx cell lines expressing gene (s) of interest … open shelf bookcase room dividerWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I... open shelf changing tableWebHow to improve transfection efficiency of Flp-In 293 T-REx cells in order to create a stable cell line? Hi everybody, I am trying to generate stable cell line by using Flp-In 293 T-REx … open shelf garage cabinethttp://www.protocol-online.org/biology-forums-2/posts/5898.html ipaf training telfordWebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes open shelf cabinet under tableWebAs a first step to Flp-In T-rex cell lines, you have to create the so called host cell line, which is generated through stable random integration of two plasmids pFRT/lacZeo … ipaf training testWebApr 1, 2024 · Protocol used to generate stable Flp-In T-REx-HEK 293 cell l ines expressing WT or . mutant GCase (E326K o r L444P) as. a V5-FLAG-tagged protein using a tetracycline-inducible system. open shelf credenza